Galactose and its metabolites disrupt spindle structure in metaphase II mouse oocytes
نویسندگان
چکیده
منابع مشابه
Spindle and chromosomal alterations in metaphase II oocytes.
The spindle apparatus is a vital structure and must be structurally intact for proper segregation of the oocyte's genetic material during metaphase II. Endometriosis, oxidative stress, and cryopreservation can all adversely affect the structural integrity of the spindle, potentially resulting in aneuploidy and spontaneous abortion of the embryo. Advances in spindle imagery have made it possible...
متن کاملNon-spindle microtubule organizing centers in metaphase II-arrested mouse oocytes
A human autoantiserum (5051) directed against pericentriolar material (PCM) was used to study the distribution of microtubule-organizing centers (MTOCs) in the oocyte and during the first cell cycle of mouse development. In oocytes, the PCM was found not only at the poles of the barrel-shaped metaphase II spindle but also at many discrete loci around the cytoplasm near the cell cortex. The spin...
متن کاملP-103: Polscope Analysis of Meiotic Spindle and Zona Pellucida Birefringence of Metaphase II Oocytes in Polycystic Ovarian Syndrome Patients
Background Polycystic ovarian syndrome (PCOs) is one of the most common causes of infertility in women of reproductive age. Currently, one of the best therapeutic options for PCOs patients is ICSI. Moreover, IVM can be a useful technique for women with PCOs who are at the risk of ovarian hyperstimulation syndrome (OHSS). On the other hand, the oocyte quality can be a determining factor for outc...
متن کاملAge-Associated Lipidome Changes in Metaphase II Mouse Oocytes
The quality of mammalian oocytes declines with age, which negatively affects fertilization and developmental potential. The aging process often accompanies damages to macromolecules such as proteins, DNA, and lipids. To investigate if aged oocytes display an altered lipidome compared to young oocytes, we performed a global lipidomic analysis between oocytes from 4-week-old and 42 to 50-week-old...
متن کاملMetaphase I Arrest upon Activation of the Mad2-Dependent Spindle Checkpoint in Mouse Oocytes
BACKGROUND The importance of mitotic spindle checkpoint control has been well established during somatic cell divisions. The metaphase-to-anaphase transition takes place only when all sister chromatids have been properly attached to the bipolar spindle and are aligned at the metaphase plate. Failure of this checkpoint may lead to unequal separation of sister chromatids. On the contrary, the exi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Fertility and Sterility
سال: 2015
ISSN: 0015-0282
DOI: 10.1016/j.fertnstert.2015.07.170